The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Defective smooth muscle development in qkI-deficient mice.<br>Dev. Growth Differ., 45, 449-462 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC. <br>Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. B6;129P2-Qk<tm1Imeg>/Rbrc RBRC00820 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Defective smooth muscle development in qkI-deficient mice.<br>Dev. Growth Differ., 45, 449-462 (2003). <br>寄託後１年間は、共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。 Qk (quaking) gene knockout mice. The initiation codon and first 40 amino acids of a highly conserved QUA1 domain of Qk gene were replaced with an IRES and a betageo fusion gene. Homozygous mutant mice die in utero E9.5 and E10.5. E14.1 [129P2/OlaHsd] Kuniya ABE qkI Knockout, qkI遺伝子のノックアウトマウス, TgH(qkI)2Imeg 理化学研究所筑波研究所 C (3-6 months) qkI Knockout mouse Fluorescent Proteins/lacZ System <a href='https://brc.riken.jp/mus/pcr00820'>Genotyping protocol -PCR-</a> TgH(qkI)2Imeg 阿部　訓也 qkI Knockout, qkI遺伝子のノックアウトマウス Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://mcd.brc.riken.jp/en/" target="_blank">Lab HP</A> Qk (quaking) 遺伝子のノックアウトマウス。Qk遺伝子の開始コドンとQUA1ドメインの保存性の高い最初の40アミノ酸領域をIRES-betageo遺伝子で置換。ホモマウスは胎生期9.5〜10.5日に致死となる。 C（3〜6か月） Developed by Kuniya Abe and Ken-ichi Yamamura, Insitute of Molecular Embryology and Genetics, Kumamoto University. E14.1 ES cells were used for generation of the knockout mice. The mutant mice were crossed to C57BL/6J. true Sibling Mating (Heterozygote X Wild)Heterozygote RIKEN,Tsukuba Sibling Mating (Heterozygote X Wild)Heterozygote Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), beta-geo(E. coli lacZ, E. coli neo), SV40 poly A, mouse qkI genomic DNA 熊本大学発生医学研究センター・阿部訓也・山村研一。E14.1 ES細胞を用いて作出。 qkI Knockout mouse, TgH(qkI)2Imeg